abstract：:In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...

journal_title：Journal of microbiology and biotechnology

authors： Park HJ,Min J,Ahn JM,Cho SJ,Ahn JY,Kim YH

更新日期：2015-02-01 00:00:00

abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Xu Y,Chen X,Zhang LY

更新日期：2015-01-01 00:00:00

abstract：:Open raceway ponds are cost-efficient for mass cultivation of microalgae compared with photobioreactors. Although low-cost options like wastewater as nutrient source is studied to overcome the commercialization threshold for biodiesel production from microalgae, a cost analysis on the use of wastewater and other incre...

journal_title：Journal of microbiology and biotechnology

authors： Kang Z,Kim BH,Ramanan R,Choi JE,Yang JW,Oh HM,Kim HS

更新日期：2015-01-01 00:00:00

abstract：:The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNAdependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular ...

journal_title：Journal of microbiology and biotechnology

authors： Kim JH,Rhee JK,Ahn DG,Kim KP,Oh JW

更新日期：2014-12-28 00:00:00

abstract：:Metarhizium anisopliae is a widely studied model to understand the virulence factors that participate in pathogenicity. Proteases such as subtilisin-like enzymes (Pr1) and trypsin-like enzymes (Pr2) are considered important factors for insect cuticle degradation. In four M. anisopliae strains (798, 6342, 6345, and 634...

journal_title：Journal of microbiology and biotechnology

authors： Rosas-García NM,Avalos-de-León O,Villegas-Mendoza JM,Mireles-Martínez M,Barboza-Corona JE,Castañeda-Ramírez JC

更新日期：2014-11-28 00:00:00

abstract：:Although most algae cultivation systems are operated in suspended culture, an attached growth system can offer several advantages over suspended systems. Algal cultivation becomes light-limited as the microalgal concentration increases in the suspended system; on the other hand, sunlight penetrates deeper and stronger...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Oh HM,Jo BH,Lee SA,Shin SY,Kim HS,Lee SH,Ahn CY

更新日期：2014-11-28 00:00:00

abstract：:In the past, reactive oxygen species (ROS) have been considered a harmful byproduct of aerobic metabolism. However, accumulating evidence implicates redox homeostasis, which maintains appropriate ROS levels, in cell proliferation and differentiation in plants and animals. Similarly, ROS generation and signaling are in...

journal_title：Journal of microbiology and biotechnology

authors： Kim HJ

更新日期：2014-11-28 00:00:00

abstract：:Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...

journal_title：Journal of microbiology and biotechnology

authors： Kim JJ,Kwon YK,Kim JH,Heo SJ,Lee Y,Lee SJ,Shim WB,Jung WK,Hyun JH,Kwon KK,Kang DH,Oh C

更新日期：2014-11-28 00:00:00

abstract：:A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 ...

journal_title：Journal of microbiology and biotechnology

authors： Wen C,Guo W,Chen X

更新日期：2014-10-01 00:00:00

abstract：:Dephosphocoenzyme A (CoaE) catalyzes the last step in the biosynthesis of the cofactor coenzyme A. In this study, we report the identification and application of CoaE from Stretomyces peucetius ATCC27952. After expression of coaE, the protein was found to have a molecular mass of 28.6 kDa. Purification of the His-tagg...

journal_title：Journal of microbiology and biotechnology

authors： Lee NR,Rimal H,Lee JH,Oh TJ

更新日期：2014-09-01 00:00:00

abstract：:Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and...

journal_title：Journal of microbiology and biotechnology

authors： Kim YO,Park IS,Nam BH,Kim DG,Jee YJ,Lee SJ,An CM

更新日期：2014-09-01 00:00:00

abstract：:Platelet-activating factor receptor (PAFR) plays an important role in bacterial infection and inflammation. We examined the effect of the bacterial cell wall components lipopolysaccharide (LPS) and lipoteichoic acid (LTA) from Lactobacillus plantarum (pLTA) and Staphylococcus aureus (aLTA) on PAFR expression in THP-1,...

journal_title：Journal of microbiology and biotechnology

authors： Kim H,Jung BJ,Jeong J,Chun H,Chung DK

更新日期：2014-08-01 00:00:00

abstract：:The present study shows that DR1114 (Hsp20), a small heat shock protein of the radiationresistant bacterium Deinococcus radiodurans, enhances tolerance to hydrogen peroxide (H2O2) stress when expressed in Escherichia coli. A protein profile comparison showed that E. coli cells overexpressing D. radiodurans Hsp20 (EC-p...

journal_title：Journal of microbiology and biotechnology

authors： Singh H,Appukuttan D,Lim S

更新日期：2014-08-01 00:00:00

abstract：:Succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde (SSA) into succinic acid in the final step of γ-aminobutyric acid degradation. Here, we characterized Bacillus subtilis SSADH (BsSSADH) regarding its cofactor discrimination and substrate inhibition. BsSSADH showed similar val...

journal_title：Journal of microbiology and biotechnology

authors： Park SA,Park YS,Lee KS

更新日期：2014-07-01 00:00:00

abstract：:This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Sung JY,Cho HH,Kwon KC,Koo SH

更新日期：2014-06-28 00:00:00

abstract：UNLABELLED:We have previously shown that paraquat (PQ)-induced oxidative stress causes dramatic damage in various human cell lines. Naringenin (NG) is an active flavanone, which has been reported to have beneficial bioactivities, including antioxidative, anti-inflammatory, and antitumorigenic activities, with a relativ...

journal_title：Journal of microbiology and biotechnology

authors： Podder B,Song HY,Kim YS

更新日期：2014-05-01 00:00:00

abstract：:Doenjang, a traditional Korean fermented soybean paste, is made by mixing and ripening meju with high salt brine (approximately 18%). Meju is a naturally fermented soybean block prepared by soaking, steaming, and molding soybean. To understand living bacterial community migration and the roles of bacteria in the manuf...

journal_title：Journal of microbiology and biotechnology

authors： Jeong DW,Kim HR,Jung G,Han S,Kim CT,Lee JH

更新日期：2014-05-01 00:00:00

abstract：:Clostridium difficile causes mucosal damage and diarrhea by releasing two exotoxins: toxin A and toxin B. C. difficile colitis is associated with alterations in bowel flora and the failure to mount an effective antibody response. The aim of the current study was to investigate whether antitoxin sera prevent toxin-A-in...

journal_title：Journal of microbiology and biotechnology

authors： Kim DH,Lee IH,Nam ST,Nam HJ,Kang JK,Seok H,Hwang JS,Kim H

更新日期：2014-05-01 00:00:00

abstract：:Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possibl...

journal_title：Journal of microbiology and biotechnology

authors： Jung JY,Lee SR,Kim S,Chi SW,Bae KH,Park BC,Kim JH,Park SG

更新日期：2014-05-01 00:00:00

abstract：:We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detectio...

journal_title：Journal of microbiology and biotechnology

authors： Ji GY,Song HG,Son BR,Hong SB,Kim JW,Shin KS

更新日期：2014-03-28 00:00:00

abstract：:Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ΔluxS (ECM101) and ΔluxSΔrelAΔspoT (ECM201...

journal_title：Journal of microbiology and biotechnology

authors： Oh KH,Cho SH

更新日期：2014-03-28 00:00:00

abstract：:Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their vi...

journal_title：Journal of microbiology and biotechnology

authors： Zhang J,Zhang X,Zhang L,Zhao Y,Niu C,Yang Z,Li S

更新日期：2014-02-28 00:00:00

abstract：:Microbial community shifts, associated with performance data, were investigated in an anaerobic batch digester treating high-solid food waste under mesophilic conditions using, a combination of molecular techniques and chemical analysis methods. The batch process was successfully operated with an organic removal effic...

journal_title：Journal of microbiology and biotechnology

authors： Yi J,Dong B,Xue Y,Li N,Gao P,Zhao Y,Dai L,Dai X

更新日期：2014-02-28 00:00:00

abstract：:The gene encoding α-L-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Lee YE

更新日期：2014-02-28 00:00:00

abstract：:Tannase (Tan410) from a soil metagenomic library was immobilized on different supports, including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and was further characterized. The optimum pH of the immobiliz...

journal_title：Journal of microbiology and biotechnology

authors： Yao J,Chen Q,Zhong G,Cao W,Yu A,Liu Y

更新日期：2014-01-01 00:00:00

abstract：:While searching for lactic acid bacteria that can restore aging-impaired immune responses, we isolated the Toll-like receptor (TLR) 2/NF-kappaB-activating strain Lactobacillus plantarum HY7712 from kimchi and investigated its immunomodulating effect in whole-body γ-irradiated mice. Exposure to HY7712 strongly activate...

journal_title：Journal of microbiology and biotechnology

authors： Lee H,Ahn YT,Park SH,Park DY,Jin YW,Kim CS,Sung SH,Huh CS,Kim DH

更新日期：2014-01-01 00:00:00

abstract：:Citrobacter sp. is a cause of significant opportunistic nosocomial infection and is frequently found in human and animal feces, soil, and sewage water, and even in industrial waste or putrefaction. Biofilm formation is an important virulence trait of Citrobacter sp. pathogens but the process and characteristics of thi...

journal_title：Journal of microbiology and biotechnology

authors： Zhou G,Li LJ,Shi QS,Ouyang YS,Chen YB,Hu WF

更新日期：2013-12-01 00:00:00

abstract：:During the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. Discovery of oxidoreductases that can convert 3- hydroxypropionaldehyde to 1,3-propanediol using NADP...

journal_title：Journal of microbiology and biotechnology

authors： Ma CW,Zhang L,Dai JY,Xiu ZL

更新日期：2013-12-01 00:00:00

abstract：:Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this st...

journal_title：Journal of microbiology and biotechnology

authors： Tang H,Hou J,Shen Y,Xu L,Yang H,Fang X,Bao X

更新日期：2013-11-28 00:00:00

abstract：:An agar-degrading bacterium, designated as strain G7(T), was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7(T) is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed t...

journal_title：Journal of microbiology and biotechnology

authors： Chi WJ,Park JS,Kwak MJ,Kim JF,Chang YK,Hong SK

更新日期：2013-11-28 00:00:00

abstract：:Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both...

journal_title：Journal of microbiology and biotechnology

authors： Sun YT,Zhao L,Ye ZY,Fan L,Liu XP,Tan WS

更新日期：2013-11-28 00:00:00

abstract：:Different endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxol-producing endophytic fungi. Taxol extracted from...

journal_title：Journal of microbiology and biotechnology

authors： Garyali S,Kumar A,Reddy MS

更新日期：2013-10-28 00:00:00

abstract：:Ethanol fuel production from lignocellulosic biomass is emerging as one of the most important technologies for sustainable development. To use this biomass, it is necessary to circumvent the physical and chemical barriers presented by the cohesive combination of the main biomass components, which hinders the hydrolysi...

journal_title：Journal of microbiology and biotechnology

authors： Souza AC,Carvalho FP,Silva e Batista CF,Schwan RF,Dias DR

更新日期：2013-10-28 00:00:00

abstract：:The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monocl...

journal_title：Journal of microbiology and biotechnology

authors： Rodrigues ME,Costa AR,Fernandes P,Henriques M,Cunnah P,Melton DW,Azeredo J,Oliveira R

更新日期：2013-09-28 00:00:00

abstract：:Particle size and metal species are important to both soil microbial toxicity and phytotoxicity in the soil ecosystem. The effects of CuO and ZnO nanoparticles (NPs) and microparticles (MPs) on soil microbial toxicity, phytotoxicity, and bioaccumulation in two crops (Cucumis sativus and Zea mays) were estimated in a s...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Sin H,Lee S,Lee I

更新日期：2013-09-28 00:00:00

abstract：:The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex...

journal_title：Journal of microbiology and biotechnology

authors： Bideshi DK,Waldrop G,Fernandez-Luna MT,Diaz-Mendoza M,Wirth MC,Johnson JJ,Park HW,Federici BA

更新日期：2013-08-01 00:00:00

abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...

journal_title：Journal of microbiology and biotechnology

authors： Long D,Tang X,Cai K,Chen G,Shen C,Shi J,Chen L,Chen Y

更新日期：2013-08-01 00:00:00

abstract：:We isolated and functionally characterized the α- and β- subunits (ApCpnA and ApCpnB) of a chaperonin from Aeropyrum pernix K1. The constructed vectors pET3d- ApCpnA and pET21a-ApCpnB were transformed into E. coli Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) cells. The expression of ApCpnA (60.7 kDa) and ApCpnB (61.2...

journal_title：Journal of microbiology and biotechnology

authors： Lee JW,Kim SW,Kim JH,Jeon SJ,Kwon HJ,Kim BW,Nam SW

更新日期：2013-06-28 00:00:00

abstract：:Anthrax is a bacterial disease caused by the aerobic sporeforming bacterium Bacillus anthracis, which is an important pathogen owing to its ability to be used as a terror agent. B. anthracis spores can escape phagocytosis and initiate the germination process even in antimicrobial conditions, such as oxidative stress. ...

journal_title：Journal of microbiology and biotechnology

authors： Kim SH,Kim SK,Jung KH,Kim YK,Hwang HC,Ryu SG,Chai YG

更新日期：2013-06-28 00:00:00

abstract：:Gibberellins (GAs) are a group of phytohormones that control many developmental processes in higher plants. We report the cloning and expression pattern of gibberellin biosynthesis genes from a new GA-producing fungus, Fusarium proliferatum (strain KGL0401). These genes sequences are deposited in the National Center f...

journal_title：Journal of microbiology and biotechnology

authors： Rim SO,You YH,Yoon H,Kim YE,Lee JH,Kang MS,Kim C,Seu YB,Kim JG

更新日期：2013-05-01 00:00:00